THERAPEUTIC INVESTIGATION OF ANTIMICROBIAL PROPERTIES OF WITHANIA SOMNIFERA, TERMINALIA ARJUNA, BACOPA MONNIERI, RANUNCULUS SCELERATUS AND ACALYPHA INDICA.

Authors

  • Shailesh Solanki ,Debanjana Prasad

Abstract

The present work was carried out using root parts of five medicinal plants from interior areas of
Agrakhal of Uttrakhand and surajpur of Uttar Pradesh, India in order to collect the information of
these medicinal plants used traditionally. The objective of this work was to investigate and
compare, phytochemical analysis, qualitatively, antimicrobial screening activity of root extractsof
Withania somnifera, Terminalia arjuna, Bacopa monnieri, Ranunculus sceleratus andAcalypha
indicaby using solvents Ethanol, Methanol and Chloroform. In Qualitative Analysis, major active
compounds were determined. Antimicrobial screening including Minimum Inhibitory
Concentration was evaluated by Disc Diffusion method with four standard antibacterial agents
Ciprofloxacin, Tobramycin, Streptomycin, Azithromycin and two standard antifungal agents
Fluconazole, Itraconazole. Qualiitative Screening results showed, high presence of beneficial active
compounds including alkaloids, coumarin, flavonoids, phenols, tannins in ethanol and methanol
extracts. Disc Diffusion Assay revealed maximum potential antimicrobial activity at concentration
10mg/ml against microbes Bacillus subtilis(MTCC 441):18mm-24.9mm, Staphylococcus
aureus(MTCC 96):17mm-19.9mm, Pseudomonas aeruginosa (MTCC 647):16mm-18.3mm, Acinetobactorbaumanni(MTCC 1425):17.1mm-27.1mm, Saccharomyces cerevisiae(MTCC
250):15.9mm-22.2mmand Aspergillus niger(MTCC 9652):14.8mm-20.3mm were exhibited by
these extracts. The MIC was carried out at different concentrations (10mg/ml, 5mg/ml, 2.5mg/ml,
1.25mg/ml, 0.0625mg/ml). The MIC values of ethanolic and methanolic root extracts were noted to
have better function at low MIC values started at 0.625mg/ml against bacteria and 1.25mg/ml
against fungi and yeast, respectively as compared with standard agents. It was reported, these
ethanol-methanol root extractsare highly effective the for high potential medicinal properties

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Published

2020-11-03

How to Cite

Shailesh Solanki ,Debanjana Prasad. (2020). THERAPEUTIC INVESTIGATION OF ANTIMICROBIAL PROPERTIES OF WITHANIA SOMNIFERA, TERMINALIA ARJUNA, BACOPA MONNIERI, RANUNCULUS SCELERATUS AND ACALYPHA INDICA. PalArch’s Journal of Archaeology of Egypt / Egyptology, 17(6), 10017 - 10037. Retrieved from http://mail.palarch.nl/index.php/jae/article/view/2566